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    Chromatography and Blotting Papers

    Chromatography Papers

    Chromatography Papers
    • Complete line of chromatography, electrophoresis and blotting papers in various sizes
    • Produced from high quality linters, no additives, high purity, high quality and consistency
    • Low ash content - almost total absence of metallic contaminants that could lead to interferences
    • Papers have perfect structure and regularity in distribution of fibres allowing for perfect uniformity
    • Undergo rigorous quality control during manufacturing to ensure uniformity within the grade
    • Correct storage guarantees good behaviour later in the laboratory
    • Product line includes standard cellulose and ion exchange grades
    • Available in many grades with different thicknesses and flow rate characteristics
    • Family of absorbent papers of different weights and thickness also available
    • Used for blotting procedures such as transport of samples, absorption of liquids in laboratories and protection of surfaces
    Blotting Papers
    • Blotting paper available in sheets precisely cut to the most popular gel and transfer membrane sizes
    • Have a perfect level of resistance to the moist stage without fibres loosening
    • Support uniform wicking capability and capillary action for even transfers during blotting
    • Applications: teaching, organic chemistry (separation of ions difficult to identify), research biochemistry
    • In biochemistry, components in samples of nucleic acids and protein can be identified
      • By blotting and electroblotting by moist (tampon deposit) or semi dry method
      • In combination with cellulose nitrate or nylon membranes (southern, northern and western blots, electroblotting and electrophoresis)
      • For the separation of substances from a mixture according to the principle of distribution by velocities of aspiration
      • Determined by size of molecules, coefficients of distribution and molecular tools
    • In medicine the process of blotting is used to detect the presence of disease-related proteins or viruses (e.g BSE or HM)
    • Made from high purity alpha cotton cellulose, using ultra-pure RO water and no additives
    • Important parameters: Weight, thickness and speed of aspiration
    • Thickness guarantees high load of solutes
    • Speed of capillary aspiration does not guarantee better definition therefore a slower paper will always have a better resolution

    Johnson Test Papers offers a complete line of high quality chromatography, electrophoresis and blotting papers for use in gel transfer applications and chromatography techniques. These products are available in many grades with different thickness and flow rate characteristics, optimised for your specific application and come in a variety of sheets, reel, and circle sizes.

    These uniform, smooth, white sheets are produced from the specially selected, highest quality cotton linters with no additives and are widely used around the world, thanks to the purity, high quality, high value standard and consistency. This guarantees low ash content and the almost total absence of metallic contaminants, especially of Fe and Cu, which could lead to interferences.These qualities are relied upon by chromatographers and are essential to successful, reproducible chromatography. The raw materials used give these papers perfect structure and regularity in the distribution and position of the fibres, allowing perfect uniformity of the physical properties of each grade.

    They undergo rigorous quality control when being manufactured, for characteristics important to the chromatographer and are tested specifically for chromatographic techniques to ensure uniformity within the grade. Likewise, correct storage of the paper guarantees its good behaviour later in the laboratory. The chromatography paper product line includes standard cellulose and ion exchange grades. Johnson Test Papers’ family of absorbent papers come in different weights in grams and thickness for blotting procedures such as transport of samples, absorption of liquids in laboratories and protection of surfaces.

    Blotting Papers

    All types of blotting paper are available in sheets precisely cut to the most popular gel and transfer membrane sizes, allowing ‘out-of-the-box’ usage and eliminating sheet-to-sheet variations.

    Our pure cellulose blotting papers are manufactured from the highest quality cotton linters using ultra pure RO water with no additives to ensure that no contamination will occur during the transfer steps. This high purity alpha cotton cellulose reduces background noise and interference in the detection system and has a perfect level of resistance to the moist stage, without the fibers loosening.The filter papers are manufactured and tested specifically for chromatographic and blotting techniques. This supports the wicking capability and uniformity of capillary action that is important for obtaining clean and even transfers during blotting.

    The applications are very extensive: teaching, organic chemistry (separation of ions difficult to identify), research and biochemistry. In the field of biochemistry, its application is emphasised in the identification of components in samples of nucleic acids (DNA and RNA) and proteins by means of techniques of transfer by blotting and electroblotting. Usual methods by the moist (tampon deposit) or semi-dry method, in combination with cellulose nitrate or nylon membranes (Southern, Northern and Western blots, electroblotting and electrophoresis) are used for the separation of substances from a mixture according to the principle of distribution by velocities of aspiration. This is determined by the different sizes of the molecules, coefficients of distribution and molecular loads. In medicine, the process of blotting is used to detect the presence of disease-related proteins or viruses (e.g BSE or HM).

    The most important parameters that indicate to us the properties of paper for chromatography are the weight in grams, the thickness and the speed of aspiration. While a paper of greater weight in grams and thickness guarantees a high load of solutes, the speed of capillary aspiration does not guarantee better definition but the contrary. For this reason, a slow paper will always have a greater resolution.

    Applications

    • Determination of absorption of water in the production of sized paper according to the Cobb method
    • Testing of the formation of sheets in the cellulose paste industry
    • Separation and identification des additives in food
    • Determination of malic acid content in wines caused by fermentation
    • Separation and identification of samples unknown composition in research laboratories
    • Identification of components in samples of nucleic acids and proteins according to blotting and electroblotting techniques.
    • Analysis of proteins in serums.
    • Customary operations in chromatography.
    • Transport of samples.
    • Absorption of spilled liquids
    • Absorption of the excess of ink in the graphics industry
    • Reduction of the risk of rupture glass material on surface of laboratories
    • Jobs involving restoration work in the museum workshops.
    • Determination of the power of retention of water of mortars in paste.

    Key Features

    • Controlled absorption
    • Uniform absorption
    • Consistency across the sheet - Fibres predominantly in one direction
    • Batch to batch consistency
    • High purity, no sizing or fillers
    • Excellent wet-strength and handling
    • No hard spots or density variations
    • No airborne fibers
    • Custom cut
    • Bulk packaging
    • Made of pure cotton linters with a 100% α-cellulose content.
    • Thicker papers allow separation of higher quantities
    • Lower capillary rise more suitable for higher resolution
    • Made of absolutely pure raw materials, without any additive. Therefore no contamination danger during transfer steps.
    • The analytical method is similar to chromatography papers, to ensure high consistent capillary force and an equable transfer.